Journal: Cells

Article Title: Phospholipid Encapsulation of an Anti-Fibrotic Endopeptide to Enhance Cellular Uptake and Myocardial Retention

doi: 10.3390/cells12121589

Figure Lengend Snippet: L-Ac-SDKP bioavailability after daily i.p. injection in mice with and without left chest radiation. Biodistribution of Ac-SDKP in the serum and heart was evaluated after four i.p. injections given daily one day after thoracic irradiation. ( a ), representative IVIS spectrum images ( b ), and biofluorescence quantification ( c ) of the tissues demonstrating fluorescence intensity in animals receiving L-Ac-SDKP with and without radiation normalized to that in PBS-treated (vehicle) controls. The coloring corresponds to the spectrum gradient bar for the radiant efficiency unit (p/s/cm 2 /sr). Scale bar, 5 mm.

Article Snippet: The biodistribution of the fluorescent compound was analyzed using the IVIS Spectrum in vivo imaging system (PerkinElmer) in a smaller subset of animals exposed to single dose chest radiation as described before [ ].

Techniques: Injection, Irradiation, Fluorescence

Journal: mBio

Article Title: The PTEX Pore Component EXP2 Is Important for Intrahepatic Development during the Plasmodium Liver Stage

doi: 10.1128/mbio.03096-22

Figure Lengend Snippet: EXP2 knockdown severely impacts a liver-stage bioluminescence developmental reporter. (A) Representative IVIS image of NanoLuc luminescence in Huh7 cultures 48 hpi with EXP2 glmS and EXP2 ctrl . GlcN was introduced to cultures at the indicated concentrations and times. Atovaquone (ATQ; 10 nM) was added at 0 hpi as a control for parasite death. (B) Quantification of luminescence in EXP2 glmS and EXP2 ctrl (red and blue bars, respectively). Data were normalized to untreated (−GlcN) and ATQ controls (used to define 100% and 0% NanoLuc activity, respectively, in each experiment). n = 3 to 5 independent biological replicates. Error bars indicate SD. P values were determined by an unpaired, two-sided Student’s t test.

Article Snippet: To monitor development in attached cells, 48-hpi liver-stage cultures were washed with PBS and lysed in Nano-Glo luciferase assay buffer containing Nano-Glo luciferase assay substrate diluted 1:200, and luminescence was measured after 3 min on an IVIS SpectrumCT imaging system (PerkinElmer).

Techniques: Knockdown, Control, Activity Assay

Journal: mBio

Article Title: The PTEX Pore Component EXP2 Is Important for Intrahepatic Development during the Plasmodium Liver Stage

doi: 10.1128/mbio.03096-22

Figure Lengend Snippet: EXP2 knockdown reduces the formation of detached merosomes. (A) Representative IVIS image of NanoLuc luminescence in Huh7 culture supernatants at 65 hpi with EXP2 glmS and EXP2 ctrl . GlcN was introduced into cultures at the indicated concentrations at 0 hpi. Atovaquone (ATQ; 10 nM) was added at 0 hpi as a control for parasite death. (B) Quantification of luminescence in EXP2 glmS and EXP2 ctrl (red and blue bars, respectively). Data were normalized to untreated (−GlcN) and ATQ controls (used to define 100% and 0% NanoLuc activity, respectively, in each experiment). n = 3 independent biological replicates. Error bars indicate SD. P values were determined by an unpaired, two-sided Student’s t test.

Article Snippet: To monitor development in attached cells, 48-hpi liver-stage cultures were washed with PBS and lysed in Nano-Glo luciferase assay buffer containing Nano-Glo luciferase assay substrate diluted 1:200, and luminescence was measured after 3 min on an IVIS SpectrumCT imaging system (PerkinElmer).

Techniques: Knockdown, Control, Activity Assay